Journal: Cell

Article Title: Genomic nucleosome organization reconstituted with pure proteins

doi: 10.1016/j.cell.2016.09.045

Figure Lengend Snippet: Key Resources Table

Article Snippet: BSA , Biorad , 500-0002.

Techniques: Recombinant, Magnetic Beads, Gel Extraction, Bradford Assay, Sequencing, Plasmid Preparation, Software

Journal: eLife

Article Title: Generation of human hepatic progenitor cells with regenerative and metabolic capacities from primary hepatocytes

doi: 10.7554/eLife.47313

Figure Lengend Snippet: ( A ) hALB levels in blood of cDNA-uPA/SCID mice. Each line indicates the level in an individual mouse. Colors denote the passage number of transplanted hCLiPs. ( B ) Representative images of cDNA-uPA/SCID mouse livers highly (left and middle panels) and slightly (right panel) repopulated by hCLiPs. The percentages indicate RIs. ( C ) Distribution of RIs in livers of cDNA-uPA/SCID mice at 10–11 weeks after transplantation of hCLiPs, as assessed by IHC of CYP2C (shown in B). Colors denote the passage number of transplanted hCLiPs. RIs were calculated for samples marked by asterisks using hepatocytes isolated from chimeric livers by two-step collagenase perfusion followed by incubation with magnetic beads conjugated with a specific anti-mouse antibody (see Materials and methods for details). Bars indicate the mean ± SEM. ( D ) hALB levels in sera of TK-NOG mice. Each line indicates the level in an individual mouse. ( E ) Representative IHC of human CYP2C in TK-NOG mouse livers highly (left panel) and intermediately (right panel) repopulated by hCLiPs. The percentages indicate RIs determined based on this IHC. ( F ) A dot plot showing the distribution of RIs in livers of cDNA-uPA/SCID mice at 10–11 weeks after transplantation of hCLiPs. Bars indicate the mean ± SEM. ( G ) IHC of the hepatic function marker proteins MDR1 (left panels) and TTR (right panels). Sections were counterstained with an anti-human mitochondria antibody (green) and DAPI. Images of sections transplanted with hCLiPs derived from lot FCL are shown as representative data. ( H ) IHC of the zone 3-specific protein GLUL. Sections were counterstained with an anti-human mitochondria antibody (green) and DAPI. Images of sections transplanted with hCLiPs derived from lot FCL are shown as representative data. ( I ) IHC of the zone 3-specific CYPs CYP1A2 and CYP3A4. Sections were counterstained with an antibody against human CYP2C, which does not show strong zone specificity. Nuclei were also counterstained with DAPI in merged images. Images of sections transplanted with hCLiPs derived from lot FCL are shown as representative data. PV and CV indicate portal vein and central vein, respectively.

Article Snippet: From 2 weeks after transplantation, approximately 20 μl blood was collected each week from the tail and the hALB concentration was measured using a Human Albumin ELISA Quantitation Kit (Bethyl, Montgomery, TX).

Techniques: Transplantation Assay, Isolation, Incubation, Magnetic Beads, Marker, Derivative Assay

Journal: eLife

Article Title: Generation of human hepatic progenitor cells with regenerative and metabolic capacities from primary hepatocytes

doi: 10.7554/eLife.47313

Figure Lengend Snippet: ( A ) Blood hALB levels in cDNA-uPA/SCID mice which were transplanted with FCL-P4-hCLiPs (purple lines). Each line indicates the level in an individual mouse. cDNA-uPA/SCID mice transplanted with FCL-P0-hCLiPs (red lines) or FCL-P1-hCLiPs (a green line), which were successfully repopulated with high RIs (>60%), are shown as reference (these data are used also in ). ( B ) Representative images of cDNA-uPA/SCID mouse livers transplanted with FCL-P4-hCLiPs. The liver tissue was harvested 8 weeks after transplantation, and stained with a human-specific CYP2C antibody to estimate the RIs. ( C ) Serum hALB levels in TK-NOG mice which were transplanted with FCL-P3-hCLiPs with (light blue lines) or without (green lines) hepatic induction. Each line indicates the level in an individual mouse. TK-NOG mice transplanted with FCL-P0-hCLiPs (red lines) are shown as reference (these data are used also in ).

Article Snippet: From 2 weeks after transplantation, approximately 20 μl blood was collected each week from the tail and the hALB concentration was measured using a Human Albumin ELISA Quantitation Kit (Bethyl, Montgomery, TX).

Techniques: Transplantation Assay, Staining